Table 1. Summary of Biologic Samples Collected from PCaP Subjects
| Sample | Collection Vial | Description | Unit | Units/Subject | Vol. or Conc./Unit |
|---|---|---|---|---|---|
| Serum | Red Top* | No Additive | aliquot | 10 | ~0.5 ml |
| Plasma | Lavender Top | EDTA | aliquot | 6 | ~0.3 – 0.5 ml |
| Yellow Top | ACD | aliquot | 6 | ~1.0 ml | |
| RBCs | Lavender Top | crude packed (WBCs) | aliquot | 2 | ~1.0 ml |
| Yellow Top | Ficol purified (RBCs only) | aliquot | 2 | ~1.5 ml | |
| Immortalized PBMCs | Yellow Top | EBV transformed B-cells | aliquot | 6 | 1.0 ml 3-5 x 106 cells/ml |
| DNA | Yellow Top – PB | Genomic-peripheral blood | aliquot | 7 | 50µl@100ng/µl [5µg] |
| 7 | 100µl@100ng/µl [10µg] | ||||
| 1 | stock | ||||
| Yellow Top – IC | Immortalized Cell (IDNA) | aliquot | 18 | 100µl@100ng/µl [10µg] | |
| 1 | stock | ||||
| Mouth Rinse | Buccal Cell (bcDNA) | aliquot | 7 | 50µl@100ng/µl [5µg] | |
| 7 | 100µl@100ng/µl [10µg] | ||||
| 1 | stock | ||||
| Adipose (abdominal) | Needle | needle and Luer-lock | aliquot | 1 | variable |
| Urine | conical | No Additive | aliquot | 1 | 10 ml |
| Preservative (ascorbic acid) | aliquot | 1 | 10 ml | ||
| Toenails | envelope | all toenails on 1 foot | cryovial | 1 | nail clippings |
| Diagnostic Tissue | paraffin blocks | 4 micron sections | slides | 21 | 4 micron |
| Prostatectomy Tissue | paraffin blocks | punched cores of CaP | TMA | 30 slides/TMA | up to 30 sections |
| paraffin blocks | 4 micron | slides | 47 | 4 micron |
NOTE: In most cases biological samples were collected after initiation of treatment.
* a portion of red top serum tubes were transported from the field at ambient temp at UNC through April 30, 2007; the remaining UNC red top tubes and all of LSU red top tubes were transported on ice.
